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KMID : 0861020200350040025
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Korea Journal of Herbology
2020 Volume.35 No. 4 p.25 ~ p.36
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The Protective Effect of Water Extract of Phellodendri Cortexin Chronic Reflux Esophagitis-induced Rats
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Lee Se-Hui
Lee Jin-A
Shin Mi-Rae
Lee Ji-Hye
Roh Seong-Soo
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Abstract
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Objective : Reflux esophagitis is a disease caused by the reflux of gastric acid and inflammation due to unstable gastroesophageal sphincter. The aim of the present study was to clarify the effect of Phellodendri Cortex (PC) on chronic reflux esophagitis (CRE) in rats.
Methods : The anti-oxidant activity of PC was measured by total polyphenol, total flavonoid contents, 1, 1-diphenyl- 2-picrylhydrazyl (DPPH), and 2, 2’-azinobis-3-ethyl-enzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity. A CRE was established surgically in SD rats. And then CRE rats were treated with water or PC 200 ㎎/㎏ body weight for 14 days. Besides, the anti-oxidant and inflammatory protein levels were evaluated using western blotting.
Results : PC reduced esophagus tissues injury. The total polyphenol (36.05 ± 0.25 ㎎/g) and total flavonoid (72.90 ± 0.61 ㎎/g) of PC showed a high content. PC strongly reduced radical scavenging activities (DPPH IC50 43.58 ± 1.54 ㎍/㎖; ABTS IC50 36.75 ± 0.35 ㎍/㎖).
Moreover, reactive oxygen species (ROS) and peroxynitrite (ONOO-) levels in serum, the protein expression of inducible nitric oxide synthases (iNOS) were significantly reduced. In addition, the protein expression of NADPH oxidases related to oxidative stress were significantly reduced in PC compared to CRE control.
PC effectively reduced inflammatory factors including, TNF-α, and IL-6 via NF-κBp65 inactivation through the inhibition of p-IκBα and increased anti-oxidant enzyme such as HO-1, SOD, catalase, and GPx-1/2 via Nrf2 activation.
Conclusions : Taken together, these results show that PC can alleviate the esophageal mucosal ulcer though the inhibition of NF-κB inflammatory and the enhancement of Nrf2 anti-oxidant pathway.
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KEYWORD
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Phellodendri Cortex, chronic reflux esophagitis, esophageal mucosal ulcer, anti-oxidant, anti-inflammatory
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